RBRC03515, B6.Cg-Aire<tm2Mmat>/Rbrc

RBRC No. RBRC03515
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Aire<tm2Mmat>/Rbrc
Former Common name Aire(GFP) (Acc.No. CDB0479K)
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology and Mitsuru Matsumoto, Institute for Enzyme Research, University of Tokushima. TT2 ES cells were used.
Strain description Aire GFP knock-in mice. A GFP was inserted in the Aire gene locus between exon 1 and 2.
Health Report
Gene Details
Promoter No Data
1 Symbol Aire
Symbol name autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy)
Chromosome 10
Common name No Data
Symbol description No Data
2 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 10
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 10
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 10
Common name No Data
Symbol description No Data
References J Exp Med. 2008 Nov 24;205(12):2827-38. Epub 2008 Nov 17.

Aire controls the differentiation program of thymic epithelial cells in the medulla for the establishment of self-tolerance.
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
(1) The RECIPIENT shall obtain a prior agreement from Dr. Mitsuru Matsumoto (mitsuru@ier.tokushima-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0479K) (http:www.cdb.riken.jp/arg/mutant%20mice%20list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (J Exp Med. 2008 205(12):2827-38.) (4) Please inform the DEPOSITOR (mutant@cdb.riken.jp) about the paper when published.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Genotyping protocol <PCR>
Depositor RIKEN, CDB (RIKEN Center for Developmental Biology) RIKEN, CDB
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months