RBRC03556, B6.129-Dmd<tm1Mok>

RBRC No. RBRC03556
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129-Dmd<tm1Mok>
Former Common name Mdx52, B6-Dmd KO
H-2 Haplotype No Data
ES cell line CCE/EK.CCE [129S/SvEv-Gpi1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Motoya Katsuki, Institute of Medical Science, University of Tokyo. CCE/EK.CCE ES cells were used. The mutant mice were backcrossed to C57BL/6 by Naohiro Hashimoto, National Center for Geriatrics and Gerontology.
Strain description dystrophin (Dmd) gene knockout mice. The exon 52 was replaced with a neo cassette. Mutant mice show hypertrophic skeletal muscles. Homozygous female and hemizygous male mutants are viable and fertile. The mice are difficult to start breeding at 8-10 weeks (12-16 weeks is better).
Colony maintenance Homozygote (female) x Hemizygote (male) [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Dmd
Symbol name dystrophin, muscular dystrophy
Chromosome X
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (Klebsiella pneumoniae)
Chromosome X
Common name No Data
Symbol description No Data
References Biochem Biophys Res Commun. 1997 Sep 18;238(2):492-7.

Targeted disruption of exon 52 in the mouse dystrophin gene induced muscle degeneration similar to that observed in Duchenne muscular dystrophy.
Research applications Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Biochem. Biophys. Res. Commun., 238, 492-497 (1997).
Additional information
1 Mouse of the Month Apr 2014
2 Genotyping protocol <PCR>
Depositor Hashimoto, Naohiro (National Institute for Longevity Sciences) Hashimoto, Naohiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months