RBRC03559, 129.Cg-Pax7<tm2Pgr>

RBRC No. RBRC03559
Type Targeted MutationCartagena
Species Mus musculus
Strain name 129.Cg-Pax7<tm2Pgr>
Former Common name PaxZ129, 129-Pax7/lacZ
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Peter Gruss, Max Planck Institute for Biophysical Chemistry. The mutant mice were backcrossed 129 over 20 times by Naohiro Hashimoto, National Center for Geriatrics and Gerontology.
Strain description Pax7 gene knockout mice. The first exon of the paired box was replaced with a neo cassette (RBRC03558) and lacZ-neo cassette (RBRC03559), respectively. In the C57BL/6 background, most homozygous mutant mice die within 3 weeks after birth (survival rate is below 10%). On the other hand, in the 129 background (RBRC03558 and RBRC03559), approximately 50% of mutant mice die within 3 weeks after birth (details unknown). Heterozygous mutant mice for lacZ repoter Pax7<tm2Pgr> (RBRC03559) show negative X-gal staining in the muscle satellite cells.
Colony maintenance Heterozygote x Wild-type [or Crossing to 129<+Ter>/SvJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Pax7
Symbol name paired box gene 7
Chromosome 4
Common name No Data
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 4
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (Klebsiella pneumoniae)
Chromosome 4
Common name No Data
Symbol description No Data
References

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Pax7tm2Pgr allele (Peter Gruss, Max Planck Institute for Biophysical Chemistry). (http://www.mpibpc.mpg.de/de/gruss)
Additional information
1 MATERIAL TRANSFER AGREEMENT (English)
2 Genotyping protocol <PCR>
Depositor Hashimoto, Naohiro (National Institute for Longevity Sciences) Hashimoto, Naohiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months