RBRC03709, B6.B6CB-Disc1<tm1Kozo>

RBRC No. RBRC03709
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.B6CB-Disc1<tm1Kozo>
Former Common name DISC1 (delta 2-3), DISC1 KO, Disc1(-) (Acc.No. CDB0583K)
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain C57BL/6
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Keisuke Kuroda, Kozo Kaibuchi, Nagoya University, Graduate School of Medicine in 2008. A loxP/PGK-Neo-pA/loxP cassette was transferred into TT2 ES cells to replace most of exon 2 and all of exon 3 of Disc1 gene. The mutant mice were backcrossed to C57BL/6J.
Strain description Disc1 knockout mice. Most of exon 2 and all of exon 3 of Disc1 gene were replaced with a loxP/PGK-Neo-pA/loxP cassette. Homozygous mutant mice are viable and fertile.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJmsSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol Disc1
Symbol name disrupted in schizophrenia 1
Chromosome 8
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 8
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 8
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Hum. Mol. Genet., 20, 4666-4683 (2011).
(1) The RECIPIENT shall obtain a prior agreement from Dr. Kouzo Kaibuchi (kaibuchi@med.nagoya-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0583K) (http:www.cdb.riken.jp/arg/mutant%20mice%20list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (未定) (4) Please inform the DEPOSITOR (mutant@cdb.riken.jp) about the paper when published.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor RIKEN, CDB (RIKEN Center for Developmental Biology) RIKEN, CDB
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
The Altered Supramolecular Structure of Dopamine D2 Receptors in Disc1-deficient Mice. Sci. Rep.8(1): 1692 (2018).(29374282)
Onishi T, Sakamoto H, Namiki S, Hirose K.