RBRC03743, B6D2-Tg(CAG/Su9-DsRed2,Acr3-EGFP)RBGS002Osb

RBRC No. RBRC03743
Type TransgeneCartagena
Species Mus musculus
Strain name B6D2-Tg(CAG/Su9-DsRed2,Acr3-EGFP)RBGS002Osb
Former Common name B6D2F1-Tg(CAG/su9-DsRed2,Acr3-EGFP), RBGS002Osb (Nov. 2011), RBGS-002
H-2 Haplotype No Data
Background strain Jcl:B6D2F1
1 Appearance No Data
Genotype No Data
Strain development Developed by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. This transgenic line was generated by coinjected with Acr3-EGFP and CAG/su9-DsRed2 transgene constructs into fertilized eggs. C57BL/6 and DBA/2 mixed background.
Strain description Trangenic mice expressing GFP in sperm acrosome from acr3-EGFP and RFP in sperm mitochondoria from CAG/su9-DsRed2. The dual fluorescent sperm show normal fertilizing ability and can be observed through uterine and oviductal walls under excitation light. Acrosome reacted sperm and acrosome intact sperm are easily distinguishable from each other by red fluorescence. Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter. CAG/su9-DsRed2, in which a RFP(DsRed2) is connected to the CAG promoter with a mitochondrial import signal sequence of Atp5g1(su9).
Colony maintenance Carrier x Noncarrier [or Crossing to Jcl:B6D2F1]
Health Report
Gene Details
Promoter No Data
1 Symbol DsRed2
Symbol name Red Fluorescent Protein (Discosoma sp.)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
2 Symbol su9
Symbol name mitochondria localization signal of mouse Atp5g1[ATP synthase Fo complex subunit 9 (su9)]
Chromosome UN
Common name No Data
Symbol description No Data
Promoter acrosin promoter
3 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References Animal Experimentation, 59(1), (2009).

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Exp. Anim., 59:105-107(2010).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
2 Genotyping protocol <PCR>
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live mouse :
BRC mice in Publications
Title Journal
Unique insights into maternal mitochondrial inheritance in mice. Proc Natl Acad Sci U S A.110(32): 13038-43 (2013).(23878233)
Luo SM, Ge ZJ, Wang ZW, Jiang ZZ, Wang ZB, Ouyang YC, Hou Y, Schatten H, Sun QY.
The Behavior and Acrosomal Status of Mouse Spermatozoa In Vitro, and Within the Oviduct During Fertilization After Natural Mating. Biol. Reprod. (2016).(27417908)
Hino T, Muro Y, Tamura-Nakano M, Okabe M, Tateno H, Yanagimachi R.
LRRC8/VRAC anion channels are required for late stages of spermatid development in mice. J. Biol. Chem. (2018).(29880644)
Lück JC, Puchkov D, Ullrich F, Jentsch TJ.