RBRC03977, B6;129-Chr5-Pirc<tm1Ms>

RBRC No. RBRC03977
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129-Chr5-Pirc<tm1Ms>
Former Common name Chr5 piRNA cluster KO
H-2 Haplotype No Data
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Toshiaki Watabe and Yasuhiro Yamamoto, National Institute of Genetics in 2009. R1 ES cells were used.
Strain description Chromosome 5 piRNA cluster knockout mice. The promoter region of RNA precursor was replaced with a neo cassette. piRNA from Chr5 piRNA cluster was not generated. So far homozygous mutant mice show no obvious abnormality.
Health Report
Gene Details
Promoter No Data
1 Symbol Chr5-Pirc
Symbol name Chromosome 5 piRNA cluster
Chromosome 5
Common name No Data
Symbol description No Data
2 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 5
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 5
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 5
Common name No Data
Symbol description No Data
References No Data

Research applications Cell Biology Research,
Cre/loxP system,
Reproductive Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
後日指定
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT must contact the DEPOSITOR and DEVELOPER in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For two years form deposit, the RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR and DEVELOPER.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Sasaki, Hiroyuki (National Institute of Genetics) Sasaki, Hiroyuki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months