RBRC04200, B6.129P2-Cxcl12<tm2Tng>

RBRC No. RBRC04200
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Cxcl12<tm2Tng>
Former Common name CXCL12-GFP
H-2 Haplotype No Data
ES cell line E14.1 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takashi Nagasawa, Research Institute, Osaka Medical Center for Maternal and Child Health. E14.1 ES cells were used. The mutant mice were backcrossed to C57BL/6J for 6 times, and then maintained by sibling mating.
Strain description CXCL12-GFP knock-in mice. Exon 1 of the Cxcl12 gene was replaced with a GFP expression cassette.
Colony maintenance Heterozygote x Wild-type [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Cxcl12
Symbol name chemokine (C-X-C motif) ligand 12
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name neo; neomycin;
Symbol description No Data
References Immunity. 2003 Aug;19(2):257-67.

Long-term hematopoietic stem cells require stromal cell-derived factor-1 for colonizing bone marrow during ontogeny.
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT is requested to make a Material Transfer Agreement with the DEPOSITOR. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent form the DEPOSITOR.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Mouse of the Month Dec 2014
3 Genotyping protocol <PCR>
Depositor Nagasawa, Takashi (Institute for frontier medical sciences, Kyoto university) Nagasawa, Takashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months