RBRC04292, B6-Pold1<tm1.uchi>

RBRC No. RBRC04292
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6-Pold1<tm1.uchi>
Former Common name Pold1
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Arikuni Uchimura and Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University in 2007. C57BL/6J derived ES cells were used to generate the knockout mice. The mutant mice were crossed to C57BL/6J.
Strain description Pold1 (polymerase (DNA directed), delta 1, catalytic subunit) gene knockout mice. A loxP flanked polyA-neo cassette was inserted in intron 10. The exon 10 contains a D400A substitution. Homozygous mutant mice are embryonic lethal.
Colony maintenance Heterozygote x Wild-type [C57BL/6J(Crlj)]
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol Pold1
Symbol name polymerase (DNA directed), delta 1, catalytic subunit
Chromosome 7
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 7
Common name No Data
Symbol description No Data
References

Research applications Cell Biology Research,
Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. If the RECIPIENT’s research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Yagi, Takeshi (Graduate School of Frontier Biosciences, Osaka University) Yagi, Takeshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months