RBRC04293, B6-Pold1<exo.uchi>

RBRC No. RBRC04293
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6-Pold1<exo.uchi>
Former Common name Pold1
H-2 Haplotype No Data
ES cell line Not specified (ES cell)
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Arikuni Uchimura and Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University in 2007. C57BL/6J derived ES cells were used to generate the knockout mice. The polyA-neo cassette was removed by crossing with Sycp1-cre mice. The mutant mice were crossed to C57BL/6J.
Strain description Pold1 (polymerase (DNA directed) D400A variant mutant mice lacking 3'-5'exonuclease activity in the Pol delta. About 30% of homozygous mutant mice die with abnormally swollen thymus. Most surviving mice develop nodules on their tails. It is preferable to avoid a mating using heterozygous male across continuous generations (2 generations and up) to avoid an increasing mutation rate.
Colony maintenance Heterozygote x Wild-type [C57BL/6J(Crlj)]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Pold1
Symbol name polymerase (DNA directed), delta 1, catalytic subunit
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
References

Research applications Cell Biology Research,
Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. If the RECIPIENT’s research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR.
Additional information
1 Lab HP (Japanese)
2 Mouse of the Month Dec 2015
3 Genotyping protocol <PCR>
Depositor Yagi, Takeshi (Graduate School of Frontier Biosciences, Osaka University) Yagi, Takeshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months