RBRC04737, B6.129S2-Nmu<tm1Mko> Nms<tm1Mko>

RBRC No. RBRC04737
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129S2-Nmu<tm1Mko> Nms<tm1Mko>
Former Common name NMU/NMS-dKO
H-2 Haplotype No Data
ES cell line D3 [129S2/SvPas]
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Masayasu Kojima, Institute of Life Science, Kurume University in 2000. D3 ES cells were used to generate the mutant mice. The mice were backcrossed to C57BL/6.
Strain description Nmu (Neuromedin U) and Nmus (Neuromedin S) gene double knock-out mice. Nmu KO: Exon 9 of the Nmu gene was replaced with a PGK-neo cassette. Homozygous mutant mice show a increased body weight. Nms KO: Exon 8 of the Nms gene was replaced with a neo cassette. Homozygous mutant mice show no obvious abnormality. Nmu gene knockout mice (RBRC04549), Nms gene knockout mice (RBRC04550).
Colony maintenance Nmu: Homozygote x Homozygote [or Crossing to C57BL/6JJcl]; Nms: Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Nms
Symbol name neuromedin S
Chromosome 1
Common name No Data
Symbol description No Data
2 Symbol Nmu
Symbol name neuromedin U
Chromosome 5
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 1
Common name No Data
Symbol description No Data
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 5
Common name No Data
Symbol description No Data
References J Exp Med. 2005 Jul 18;202(2):217-24. Epub 2005 Jul 11.

The neuropeptide neuromedin U promotes inflammation by direct activation of mast cells.
The neuropeptide neuromedin U promotes inflammation by direct activation of mast cells.
Research applications Metabolism Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Because no paper has been published on neuromedin S knockout mice, the RECIPIENT is requested to contact the DEPOSITOR in publishing the research results obtained by use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Kojima, Masayasu (Kurume university) Kojima, Masayasu
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months