RBRC04807, C57BL/6N-Spata9<tm1Osb>

RBRC No. RBRC04807
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6N-Spata9<tm1Osb>
Former Common name B6-Spata9 KO
H-2 Haplotype No Data
ES cell line EGR-G101 [C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strain C57BL/6NJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hide Hatayama and Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2010.
Strain description Spata9 gene knockout mice. spata9, spermatogenesis associated 9 is a gene specifically expressing in testis.
Colony maintenance Heterozygote X Wild-type [C57BL/6NJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Spata9
Symbol name spermatogenesis associated 9
Chromosome 13
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 13
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 13
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 13
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
5 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 13
Common name No Data
Symbol description No Data
References Unpublished

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Proc. Natl. Acad. Sci. U S A., 113, 7704-7710 (2016).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months