RBRC04832, B6.129P2(CD1)-Id4<tm1Fsky>

RBRC No. RBRC04832
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2(CD1)-Id4<tm1Fsky>
Former Common name B6-Id4 KO
H-2 Haplotype No Data
ES cell line E14.1 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Fred Sblitzky, University of Nottingham in 2005. A lacZ-neo cassette replaced 220 bp of the ID4 cording region including the HLH domain. The mutant mice were backcrossed to C57BL/6 from 129 and CD1 (ICR) mixed background.
Strain description Id4 knockout mice. Id4 is a member of inhibitor of differentiation / inhibitor of DNA binding protein family. Homozygous mutant mice exhibit a abnormal brain structures.
Colony maintenance Heterozygote X Wild-type [C57BL/6JJcl] Homozygous mutant mice are small and most of them die within 8 weeks of age.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Id4
Symbol name inhibitor of DNA binding 4
Chromosome 13
Common name No Data
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome No Data
Common name No Data
Symbol description E. coli beta galactosidase
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome No Data
Common name neo; neomycin;
Symbol description No Data
References

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Dev. Biol., 280, 386-395 (2005).
The RECIPIENT must contact Prof. Fred. Sablitzky, The University of Nottingham (fred.sablitzky@nottingham.ac.uk) to obtain permission for us the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Okazaki, Yasuji (Saitama medical school research center for genomic medicine) Okazaki, Yasuji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months