RBRC04878, B6;129-Tg(CMV-EGFP,U6-shNanog)1Ttada

RBRC No. RBRC04878
Type TransgeneCartagena
Species Mus musculus
Strain name B6;129-Tg(CMV-EGFP,U6-shNanog)1Ttada
Former Common name NRi-Tg
H-2 Haplotype No Data
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Shinpei Yamaguchi and Takashi Tada, Institute for Frontier Medical Sciences, Kyoto University in 2007.
Strain description No Data
Colony maintenance Carrier x Carrier
Health Report
Gene Details
Promoter No Data
1 Symbol TATAlox
Symbol name phage P1 loxP (modified loxP site)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CMV promoter
2 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse U6 promoter
3 Symbol Nanog
Symbol name Nanog homeobox
Chromosome UN
Common name 2410002E02Rik, ecat4, ENK
Symbol description No Data
References

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Genotyping protocol <PCR>
Depositor Tada, Takashi (Institute for frontier medical sciences, Kyoto university) Tada, Takashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
NANOG Metabolically Reprograms Tumor-Initiating Stem-like Cells through Tumorigenic Changes in Oxidative Phosphorylation and Fatty Acid Metabolism. Cell Metab.23(1): 206-219 (2015).(26724859)
Chen CL, Uthaya Kumar DB, Punj V, Xu J, Sher L, Tahara SM, Hess S, Machida K.