RBRC04885, B6;129S4-Cacna1e<tm1Ttan>

RBRC No. RBRC04885
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129S4-Cacna1e<tm1Ttan>
Former Common name B6-Cacna1e KO
H-2 Haplotype No Data
Background strain No Data
1 Appearance No Data
Genotype No Data
Strain development Developed by Tsutomu Tanabe, Graduate School of Medicine, Tokyo Medical and Dental University and Tetsuo Noda, The Cancer Institute, Japanese Foundation for Cancer Research in 1997. The Cacna1e gene was disrupted by insertion of a lacZ-neo cassette.
Strain description Cacna1e knockout mice. Homozygous and heterozygous mutant mice show reduced responses to somatic inflammatory pain. Heterozygous mutant mice also show reduced respose to visceral inflammatory pain.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Cacna1e
Symbol name calcium channel, voltage-dependent, R type, alpha 1E subunit; targeted mutation 1, Tsutomu Tanabe
Chromosome 1
Common name No Data
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 1
Common name No Data
Symbol description E. coli beta galactosidase
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 1
Common name neo; neomycin;
Symbol description No Data

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Proc. Natl. Acad. Sci USA, 97, 6132-6137 (2000).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Tanabe, Tsutomu (Graduate School, Tokyo Medical and Dental University) Tanabe, Tsutomu
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
Quantitative Regional and Ultrastructural Localization of the Cav2.3 Subunit of R-type Calcium Channel in Mouse Brain. J Neurosci.32(39): 13555-13567 (2012).(23015445)
Parajuli LK, Nakajima C, Kulik A, Matsui K, Schneider T, Shigemoto R, Fukazawa Y.