RBRC04887, B6.Cg-Cacna1a<tm1.1(CACNA1A)Ttan>

RBRC No. RBRC04887
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Cacna1a<tm1.1(CACNA1A)Ttan>
Former Common name B6-Cacna1a KI#1.1
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Tsutomu Tanabe, Graduate School of Medicine in 2001. The human CACNA1 cDNA with 28 CAG repeat was inserted into mouse Cacna1a exon 1. Hygromycin cassette was remove by crossing with B6;SJL-Tg(ACTFLPe)9205Dym mice.
Strain description The human CACNA1 cDNA with 28 CAG repeat and FLAG-tag were inserted into mouse Cacna1a exon 1. Homozygous mutant mice show ataxia at around 10 days-old, and die within 3-4 weeks after birth.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Cacna1a homozygous mutant mice show ataxia at around 10 days-old, and die within 3-4 weeks after birth.
Health Report
Gene Details
Promoter No Data
1 Symbol CACNA1A
Symbol name calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (human)
Chromosome 8
Common name alpha1A, Cacnl1a4, Ccha1a, EA2, MHP1, nmf352, SCA6
Symbol description No Data
2 Symbol Cacna1a
Symbol name calcium channel, voltage-dependent, P/Q type, alpha 1A subunit
Chromosome 8
Common name alpha1A, Cacnl1a4, Ccha1a, EA2, MHP1, nmf352, SCA6
Symbol description No Data
3 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 8
Common name internal ribosomal entry site from EMCV
Symbol description No Data
4 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome No Data
Common name No Data
Symbol description No Data
References Mol Cell Neurosci. 2007 Feb;34(2):261-70. Epub 2006 Dec 22.

Properties of human Ca(v)2.1 channel with a spinocerebellar ataxia type 6 mutation expressed in Purkinje cells.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mol. Cell Neurosci., 34, 261-270 (2007).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Tanabe, Tsutomu (Graduate School, Tokyo Medical and Dental University) Tanabe, Tsutomu
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months