RBRC05143, B6.129S2-Fad158<tm1Maim>/7Maim

RBRC No. RBRC05143
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129S2-Fad158<tm1Maim>/7Maim
Former Common name B6-Fad158 KO#7
H-2 Haplotype No Data
ES cell line D3 [129S2/SvPas]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description Fad158 (Lrrc8c) knockout mice. Homozygous mutnat mice do not show body weight gain and insulin resistance by a high-fat diet. Developed by Graduate School of Pharmaceutical Sciences, Nagoya City University and Genome Information Research Center, Osaka University in 2006. A neomycin selection cassette was inserted into the exon 4 of the Fad158 gene. C57BL/6J background.
Colony maintenance Heterozygote x Wild-type
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Lrrc8c
Symbol name leucine rich repeat containing 8 family, member C
Chromosome 5
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 5
Common name No Data
Symbol description No Data
References

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol Pharm Bull. 2011 34(8):1257-63. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from Dr.Makoto Nishizuka (nishizuka@hirosaki-u.ac.jp) using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Imagawa, Masayoshi (Nagoya City University Faculty of Pharmaceutical Sciences) Imagawa, Masayoshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months