RBRC05324, 129-Sirt1<tm2.1Mcby>

RBRC No. RBRC05324
Type Targeted MutationCartagena
Species Mus musculus
Strain name 129-Sirt1<tm2.1Mcby>
Former Common name Sirt1 KI/H355Y, STOCK Sirt1<tm2.1Mcby>
H-2 Haplotype No Data
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Michael McBurney, Ottawa Regional Cancer Centre and Department of Medicine, University of Ottawa. A knock-in vector carrying a point mutation in exon 5 of Sirt1 gene, in which codon 355 was mutated from CAT (histidine) to TAT (tyrosine) was inserted into exon 5. A hygromycin resistant cassette flanked by loxP sites was removed by crossing with Prm-Cre mice (129-Tg (Prm-Cre) 580g / J).
Strain description SirT1 variant (H355Y) knock-in mice, carrying a point mutation in exon 5 of Sirt1 gene, in which codon 355 was mutated from CAT (histidine) to TAT (tyrosine). Homozygous mutant mice are infertile.
Colony maintenance Heterozygote X Wild-type
Health Report
Gene Details
Promoter No Data
1 Symbol Sirt1
Symbol name sirtuin 1 (silent mating type information regulation 2, homolog) 1 (S. cerevisiae)
Chromosome 10
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 10
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor McBurney, Michael W. (Ottawa Hospital Research Institute) McBurney, Michael W.
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Live
Live mouse :