RBRC05525, B6.B6D2-Tg(Prrx1-Sox9,-lacZ)1Haak

RBRC No. RBRC05525
Type TransgeneCartagena
Species Mus musculus
Strain name B6.B6D2-Tg(Prrx1-Sox9,-lacZ)1Haak
Former Common name Prx1-Sox9 Tg, Prx1-Sox9 transgenic, B6-Prx1-Sox9/lacZ Tg
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Haruhiko Akiyama, The University of Texas MD Anderson Cancer Center. The transgene was injected into the pronuclei of (C57BL/6 x DBA/2)F1 fertilized eggs. The mutant mice were crossed to C57BL/6.
Strain description Prx1-Sox9/lacZ transgenic mice in which Sox9 is specifically expressed in limb bud mesenchyme. Prx1-Sox9/lacZ transgene contains a 2.4 kb of the Prx1 limb specific promoter encoding the 3xHA-epitope tagged Sox9 cDNA followe by an IRES lacZ. Tg mice exhibit pre- and postaxial extra-digit formation. lacZ expression was detected in the limb buds from E9.5
Colony maintenance Carrier x Noncarrier [C57BL/6NCrSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol HA
Symbol name human influenza virus HA (hemagglutinin) tag sequence
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome UN
Common name No Data
Symbol description No Data
4 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Prx1 promoter
5 Symbol SOX9
Symbol name SRY-box containing gene 9 (human)
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Developmental Biology Research,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Matrix Biol., 26, 224-233 (2007).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Haruhiko Akiyama, Kyoto University, is requested. Depositor retains ownership of the Biological Resource, including any Biological Resource contained in any modification. Any Biological Resource delivered pursuant to the Agreement is understood to be experimental in nature and may have hazardous properties. The DEPOSITOR MAKES NO REPRESENTATIONS AND EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED. THERE ARE NO EXPRESS OR IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR THAT THE USE OF THE BIOLOGICAL RESOURCE WILL NOT INFRINGE ANY PATENT, COPYRIGHT, TRADEMARK, OR OTHER PROPRIETARY RIGHTS. In no event shall Depositor be liable for any use by recipient of the Biological Resource for any loss, claim, damage or liability, of whatsoever kind of nature, which may arise from or in connection with the use, handling or storage of the Biological Resource. Except where limited by Federal law, or by the constitution and laws of the state governing the recipient, the recipient agrees to hold harmless Depositor and The University of Texas System and their Regents, officers, agents and employees, from any liability, loss or damage they may suffer as a result of claims, demands, costs or judgments against them arising out of recipient's use of the Material and the use by recipient of the results obtained from the Research. The Biological Resource and modifications containing the Biological Resource may only be transferred to non-profit institutions for academic research purposes.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor de Crombrugghe, Benoit (The University of Texas MD Anderson Cancer Center) de Crombrugghe, Benoit
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months