RBRC05539, B6.129-Gt(ROSA)26Sor<tm1(Stop-Bmi1,EGFP)>

RBRC No. RBRC05539
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129-Gt(ROSA)26Sor<tm1(Stop-Bmi1,EGFP)>
Former Common name R26StopBmi1, B6-ROSA26/Stop-Bmi1,GFP KI
H-2 Haplotype No Data
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Atsushi Iwama, Graduate School of Medicine, Chiba University and Akihiko Koseki, RIKEN Research Center for Allergy and Immunology in 2009. The mutant mice were backcrossed to C57BL/6N.
Strain description A loxP-flanked neo<r>-stop cassette followed by FLAG-tagged Bmil, and frt-flanked IRES-EGFP cassette, and a bovine polyA sequences were knocked in the ROSA26 locus.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc]
Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 6
Common name internal ribosomal entry site from EMCV
Symbol description No Data
2 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol Bmi1
Symbol name Bmi1 polycomb ring finger oncogene
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol FLAG
Symbol name FLAG tag (synthetic)
Chromosome 6
Common name No Data
Symbol description No Data
6 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
7 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
8 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name neo; neomycin;
Symbol description No Data
References Submitted

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
未定(後日指定/will be announced)
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Genotyping protocol <PCR>
Depositor Iwama, Atsushi (Graduate School of Medical and Pharmaceutical Sciences, Chiba University) Iwama, Atsushi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months