RBRC05541, B6.129-Zfp277<tm1>

RBRC No. RBRC05541
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129-Zfp277<tm1>
Former Common name Zfp277 KO, B6-Zfp277 KO
H-2 Haplotype No Data
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Atsushi Iwama, Graduate School of Medicine, Chiba University in 2005. The mutant mice were backcrossed to C57BL/6N.
Strain description Zfp277 gene knockout mice. Exon 5 of Zfp277 gene was replaced with a floxed neo cassette. Homozygous mutant mice were born according to Menderian's law, but the growth retardation and death of homozygous mice were sporadically observed.
Colony maintenance Heterozygote x Wild-type [C57BL/6NCrSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol Zfp277
Symbol name zinc finger protein 277
Chromosome 12
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 12
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 12
Common name neo; neomycin;
Symbol description No Data
References

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
PloS One., 5, e12373 (2010).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Iwama, Atsushi (Graduate School of Medical and Pharmaceutical Sciences, Chiba University) Iwama, Atsushi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months