RBRC05550, B6.Cg-Ighm<tm1Cgn> Tg(Aicda-cre)9Mbu Gt(ROSA)26Sor<tm1Sho>

RBRC No. RBRC05550
Type Targeted Mutation and TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Ighm<tm1Cgn> Tg(Aicda-cre)9Mbu Gt(ROSA)26Sor<tm1Sho>
Former Common name muMT/Aicda-cre/R26R, B6-Ighm KO/AicdaCre/ROSA26-lacZ
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development muMT : Klaus Rajewsky, Insitute for Genetics, University of Cologne. R26R : Philippe Soriano, Mount Sinal School of Medicine. Aicda-cre : Meinrad Busslinger. Research Institute of Molecular Pathology.
Strain description This strain has Ighm knockout (Ighm<tm1Cgn>), AID-Tg (Tg(Aicda-cre)9Mbu) and ROSA26 lacZ reporter (Gt(ROSA)26Sor<tm1Sho>) alleles. Immunodeficient.
Colony maintenance Ighm : Homozygote x Homozygote ; Tg : Carrier x Carrier ; ROSA26 : Homozygote x Homozygote
Health Report
BRC facility Health Report in the last year and a half

Examination Date Room Rack
Nov 9, 2015 2-検 G
Sep 7, 2015 2-検 G
Jul 6, 2015 2-検 G
May 11, 2015 2-検 G
Mar 9, 2015 2-検 G
Jan 13, 2015 2-検 G
Nov 10, 2014 2-検 G
Sep 8, 2014 2-検 G

Gene Details
Promoter No Data
1 Symbol Ighm
Symbol name immunoglobulin heavy constant mu
Chromosome 12
Common name No Data
Symbol description No Data
2 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome No Data
Common name neo; neomycin;
Symbol description No Data
4 Symbol CD2 (human)
Symbol name CD2 (human)
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter mouse Aicda promoter
5 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References Immunity. 2008 Jun;28(6):751-62.


Instructive role of the transcription factor E2A in early B lymphopoiesis and germinal center B cell development.
Instructive role of the transcription factor E2A in early B lymphopoiesis and germinal center B cell development.
Instructive role of the transcription factor E2A in early B lymphopoiesis and germinal center B cell development.
Instructive role of the transcription factor E2A in early B lymphopoiesis and germinal center B cell development.
Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. PLoS One. 2011 6(12):e29141.
2) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
3) The BIOLOGICAL RESOURCE contains Tg(Aicda-re)9Mbu and Gt(ROSA)26Sortm1Sho. Before executing the MTA between RIKEN BRC and the RECIPIENT, the RECIPIENT shall contact Dr. Meinrad Busslinger, Research Institute of Molecular Pathology and shall execute the material transfer agreement with Research Institute of Molecular Pathology to use Tg(Aicda-cre)9Mbu. The RECIPIENT shall obtain the prior written consent of Dr. Philippe Soriano, Mount Sinai School of Medicine to use Gt(ROSA)26Sortm1Sho.
Additional information
1 Honjo Lab HP
2 Genotyping protocol <PCR>
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University) Honjo, Tasuku
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months