RBRC05642, B6.129-Hspa5<tm1Aoe>

RBRC No. RBRC05642
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129-Hspa5<tm1Aoe>
Former Common name Mutant BiP KI, B6-Hspa5 KI/HAtag
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Tomohiko Aoe, Chiba University Graduate School of Medicine in 2007. R1 ES cells were used. A floxed neo cassette was removed by crossing with CAG-Cre (B6) mice. The mutant mice were crossed to C57BL/6J.
Strain description BiP (Hspa5) gene knock-in mice. Knock-in mice expressing a HA-tag mutant BiP gene lacking the KDEL retrieval sequence. Homozygous mutant mice exhibit impaired surfactant secretion and die within a few hours after birth.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol HA
Symbol name human influenza virus HA (hemagglutinin) tag sequence
Chromosome 2
Common name No Data
Symbol description No Data
2 Symbol Hspa5
Symbol name heat shock protein 5
Chromosome 2
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 2
Common name No Data
Symbol description No Data
References Biochem Biophys Res Commun. 2008 Feb 22;366(4):1048-53. Epub 2007 Dec 26.
Cell Death Differ. 2007 Apr 20;.
Mol Cell Biol. 2008 Jan;28(1):293-301. Epub 2007 Oct 22.

Dysfunction of the ER chaperone BiP accelerates the renal tubular injury.
Dysfunction of the ER chaperone BiP accelerates the renal tubular injury.
Dysfunction of the ER chaperone BiP accelerates the renal tubular injury.
Research applications Cre/loxP system,
Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. Cell Death Differ., 14, 1475-1485 (2007). Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must contact the DEPOSITOR/DEVELOPER and notify him/her the research content. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Aoe, Tomohiko (Chiba University) Aoe, Tomohiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months