Strain Data Sheet

RBRC05642

Strain Information

Image
BRC No.RBRC05642
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Hspa5<tm1Aoe>
Former Common nameMutant BiP KI, B6-Hspa5 KI/HAtag
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Tomohiko Aoe, Chiba University Graduate School of Medicine in 2007. R1 ES cells were used. A floxed neo cassette was removed by crossing with CAG-Cre (B6) mice. The mutant mice were crossed to C57BL/6J.
Strain descriptionBiP (Hspa5) gene knock-in mice. Knock-in mice expressing a HA-tag mutant BiP gene lacking the KDEL retrieval sequence. Homozygous mutant mice exhibit impaired surfactant secretion and die within a few hours after birth.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesMol. Cell. Biol., 28, 293-301 (2008). 17954555
Cell Death Differ., 14, 1475-1485 (2007). 17464327
Biochem. Biophys. Res. Commun., 366, 1048-1053 (2008). 18158912

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hspa5heat shock protein 52Hspa5<tm1Aoe>targeted mutation 1, Tomohiko Aoe

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HAhuman influenza virus HA (hemagglutinin) tag sequence2HA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Ordering Information

Donor DNAMouse BiP gene, Human influenza hemagglutinin (HA) のHA tag 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3
Research applicationCre/loxP system
Developmental Biology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. Cell Death Differ., 14, 1475-1485 (2007). Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must contact the DEPOSITOR/DEVELOPER and notify him/her the research content. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.
DepositorTomohiko Aoe (Chiba University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data