RBRC05659, B6.Cg-Ly75<tm1.1Ksat>

RBRC No. RBRC05659
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Ly75<tm1.1Ksat>
Former Common name Cd205, B6- Ly75/DT-GFP KI
H-2 Haplotype No Data
ES cell line Bruce 4 [B6.Cg-Thy1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Katsuaki Sato, RIKEN Research Center for Allergy and Immunology in 2009. Bruce 4 ES cells derived from B6.Cg-Thy1<a> were used. The mutant mice were backcross to C57BL/6J.
Strain description IRES-DTR-EGFP cassette were inserted in to 3' untranslated region of CD205 (Ly75) gene. In mice with diphtheria toxin (DT) induced specific ablation of cd205 positive conventional dendritic cells (cDCs).
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
Health Report
Gene Details
Promoter No Data
1 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 2
Common name internal ribosomal entry site from EMCV
Symbol description No Data
2 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome 2
Common name No Data
Symbol description No Data
3 Symbol HBEGF
Symbol name heparin-binding EGF-like growth factor (human)
Chromosome 2
Common name No Data
Symbol description No Data
4 Symbol Ly75
Symbol name lymphocyte antigen 75
Chromosome 2
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 2
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System,
Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Proc. Natl. Acad. Sci. U S A, 109, 11288-11293 (2012).
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (Dr. Katsuaki Sato). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a "MTA for Siglech-IRES2DTREGFP Knock-In Micefor Academic Research Purposes” specified by the DEPOSITOR and Inserm.
Additional information
1 MTA for Cd205-IRES2DTREGFP Knock-In Mice for Academic Research Purposes (English)
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Depositor’s present address:Division of Immunology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki.
2 Genotyping protocol <PCR>
Depositor Sato, Katsuaki (RIKEN Research Center For Allergy and Immunology) Sato, Katsuaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months