RBRC05691, B6;129-Fgf18<tm1Tri>

RBRC No. RBRC05691
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129-Fgf18<tm1Tri>
Former Common name Fgf18 floxed, B6-Fgf18 floxed
H-2 Haplotype No Data
ES cell line C6 [129/SvEvTac]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Toru Imamura, National Institute of Advanced Industrial Science and Technology in 2009. A FRT-flanked PGK-neo cassette with a 5'loxP site was inserted upstream of exon 3 and a loxP site was inserted downstream of exon 3. A PGK-neo cassette was removed by crossing with FLPe mice (B6-Tg(CAG-FLPe)36 RBRC01834).
Strain description Fgf18 gene floxed mice. Exon 3 of Fgf18 gene was flanked by loxP sites. Conditional Fgf18 deficient mice can be generated by crossing with tissue-specific Cre mice. Homozygous mice are viable and fertile. Sometimes curly hairs.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6]
Health Report
BRC facility Health Report in the last year and a half

Examination Date Room Rack
Nov 11, 2013 2-検 B
Sep 9, 2013 2-検 B
Jul 8, 2013 2-検 B
May 13, 2013 2-検 B
Mar 4, 2013 2-検 B

Gene Details
Promoter No Data
1 Symbol Fgf18
Symbol name fibroblast growth factor 18
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 11
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 11
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Dermatology Research,
Developmental Biology Research,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Invest. Dermatol., 132, 1338-1345 (2012).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
For use of the BIOLOGICAL RESOURCE, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Ohmiya, Yoshihiro (National Institute of Advanced Industrial Science and Technology) Ohmiya, Yoshihiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months