RBRC05741, B6.Cg-Gzf1<Gt(Ayu21-T62*SACre)1Card>

RBRC No. RBRC05741
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Gzf1<Gt(Ayu21-T62*SACre)1Card>
Former Common name Gzf1-SACre, B6-Gzf1/Cre Gt
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Masatake Araki, Institute of Molecular Embryology and Genetics, Kumamoto University.
Strain description Gzf1-cre diver mice. The Beta-geo gene in the exchangeable gene trap clones of Gzf1 gene was replaced with a cre recombinase gene. Ubiquitous X-gal staining was observed in the pups from crossing Gzf1-cre mice with ROSA26-lacZ mice.
Colony maintenance Heterozygote x Wild-type [C57BL/6NJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol En2
Symbol name En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)
Chromosome 2
Common name No Data
Symbol description No Data
2 Symbol Gzf1
Symbol name GDNF-inducible zinc finger protein 1
Chromosome 2
Common name No Data
Symbol description No Data
3 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome 2
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 2
Common name No Data
Symbol description No Data
5 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome 2
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
6 Symbol Puro
Symbol name puromycin resistance gene (Streptomyces alboniger)
Chromosome 2
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
(will be announced)
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Araki, Masatake (Kumamoto University) Araki, Masatake
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months