RBRC05763, STOCK Izumo1<tm1Osb> Tg(Clgn-Izumo1/mCherry)Osb Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb

RBRC No. RBRC05763
Type Targeted Mutation and TransgeneCartagena
Species Mus musculus
Strain name STOCK Izumo1<tm1Osb> Tg(Clgn-Izumo1/mCherry)Osb Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb
Former Common name Izumo1 KO/Clgn-Izumo1mCherry Tg/CAGAcr-GFP Tg
H-2 Haplotype No Data
ES cell line D3 [129S2/SvPas]
Background strain Jcl:B6D2F1
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Naokazu Inoue and Dr. Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2005. C57BL/6 and DBA/2 mixed background.
Strain description Izumo1 knockout and Clgn-Izumo1/mCherry transgene double mutant mice. Izumo1 KO homozygous and Tg positive mice are fertile. The CAG/Acr-GFP transgene is also included.
Colony maintenance KO : Homozygote x Homozygote ; Clgn-Izumo1/mCherry : Carrier x Noncarrier ; CAG/Acr-GFP : Carrier x Carrier [or Crossing to Jcl:B6D2F1]
Health Report
Gene Details
Promoter No Data
1 Symbol Izumo1
Symbol name izumo sperm-egg fusion 1
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol mCherry
Symbol name Red Fluorescent Protein (Discosoma sp.)
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome No Data
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter CAG promoter (chicken beta-actin promoter, rabbit beta-globin poly A, CMV_IE enhancer), mouse proacrosin promoter, mouse proacrosin signal peptide, acrosin N_terminal peptide
5 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter human PGK promotor, human PGK poly (A)
6 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 7
Common name neo; neomycin;
Symbol description No Data
Promoter mouse calmegin promoter
7 Symbol Izumo1
Symbol name izumo sperm-egg fusion 1
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Cell Biology Research,
Cre/loxP system,
Dermatology Research,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Genotyping protocol <PCR>
Depositor Inoue, Naokazu (Osaka university)
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :