RBRC05765, B6.Cg-H2az1<tm1.1Hko> H2az2<tm1.1Hko>

RBRC No. RBRC05765
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-H2az1<tm1.1Hko> H2az2<tm1.1Hko>
Former Common name H2AZ1/2 floxed, B6-H2AZ1/2 floxed
H-2 Haplotype No Data
ES cell line M1 [(C57BL/6 x 129)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Haruhiko Koseki, RIKEN Center for Allergy and Immunology in 2009. M1 ES cells derived from (C57BL/6 x 129) F1 were used. The mutant mice were crossed to C57BL/6J. Neomycin resistant genes were removed.
Strain description H2A.Z1 and Z2 floxed mice. Histone variant H2A.Z (H2afz) gene is required for mammalian development.
Colony maintenance H2afz : Homozygote x Homozygote ; H2afv : Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol H2afv
Symbol name H2A histone family, member V
Chromosome 11
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 11
Common name No Data
Symbol description No Data
4 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 3
Common name No Data
Symbol description No Data
5 Symbol H2afz
Symbol name H2A histone family, member Z
Chromosome 3
Common name No Data
Symbol description No Data
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 3
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Koseki, Haruhiko (RIKEN Research Center For Allergy and Immunology) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months