RBRC05821, B6.Cg-Camk2a<tm1.1Oyam>

RBRC No. RBRC05821
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Camk2a<tm1.1Oyam>
Former Common name B6-CaMKII alpha (K42R), B6-Camk2a KI/K42R
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
1 Appearance No Data
Genotype No Data
Strain development Developed by Yoko Yamagata and Yuchio Yanagawa, National Institute for Physiological Sciences in 2003. Derived from TT2 ES cell line (B6CBAF1 derived). The PGK-neo cassette was removed by transient expression of cre recombinase in ES cells. C57BL/6 background (RBRC05821), ICR mixed background (RBRC05636).
Strain description Camk2a/K42R knock-in mice. Ca<2+>/calmodulin-dependent protein kinase IIalpha (CaMKII alpha/ Camk2a) is an enzyme that adds phosphates to a variety of protein substrates to modify their functions. CaMKIIalpha is especially enriched in the hippocampus, and is believed to be an essential mediator of activity-dependent synaptic plasticity and memory functions. We generated a novel kinase-dead knock-in mouse of the CaMKII alpha gene that completely and exclusively lacks its kinase activity by introducing a point mutation (K42R) that prevents ATP binding necessary for phosphorylation reaction. Both heterozygous and homozygous mice are hyperactive, but otherwise indistinguishable from wild-type mice. They are fertile and without any obvious neurological symptoms, except for sporadic observation of mild limbic seizure. Homozygous mice are severely impaired with hippocampal synaptic plasticity and hippocampus-dependent memory formation. Suitable for studies of the mechanisms of LTP and learning and memory, and as a memory-deficit animal model.
Colony maintenance Back crossed to C57BL/6NCrlCrlj for more than 20 generations.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Camk2a
Symbol name calcium/calmodulin-dependent protein kinase II alpha
Chromosome 18
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 18
Common name No Data
Symbol description No Data

Research applications Cre/loxP system,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neurosci., 29, 7607-7618 (2009).
Additional information
1 No Data
Depositor Yamagata, Yoko (National Institute for Physiological Sciences) Yamagata, Yoko
Strain Status /
(Expected delivery)

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months