RBRC05847, B6.Cg-Ednrb<tm1Ywa> Tg(DBH-Ednrb)Ywa Tg(Dct-lacZ)A12Jkn

RBRC No. RBRC05847
Type Targeted Mutation and TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Ednrb<tm1Ywa> Tg(DBH-Ednrb)Ywa Tg(Dct-lacZ)A12Jkn
Former Common name Ednrb(-/-); DBH-Ednrb; Dct-LacZ+/+, B6-Ednrb KO/DBH-Ednrb/Dct-lacZ
H-2 Haplotype No Data
ES cell line JH1 [129S7/SvEvBrd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Masashi Kato, Nagoya University Graduate School of Medicine. C57BL/6 background. Ddnrb KO and DBH-Ednrb Tg alleles were generated by Masashi Yanagisawa, Howard Hughes Medical Institute. Dct-lacZ Tg allele was generated by Ian Jackson, Western General Hospital.
Strain description Ednrb KO/DBH-Ednrb Tg/Dct-lacZ Tg mice. Ednrb homozygous and DBH-Ednrb Tg mice are viable due to a lethal megacolon phenotype of Ednrb KO mice are rescued by the DBH-Ednrb expression. These mice also express lacZ in melanocytes under the control of Dct promoter.
Colony maintenance Ednrb KO : Homozygote x Heterozygote ; DBH-Ednrb : Carrier x Carrier ; Dct-lacZ : Carrier x Carrier (Homozygote x Homozygote)
Health Report
Gene Details
Promoter No Data
1 Symbol Ednrb
Symbol name endothelin receptor type B
Chromosome 14
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name SV40 splice and polyadenylation signals
Chromosome UN
Common name No Data
Symbol description No Data
Promoter human dopamine beta hydroxylase (DBH) promoter
3 Symbol Ednrb
Symbol name endothelin receptor type B (Rat)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse dopachrome tautomerase promoter
4 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
5 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 14
Common name neo; neomycin;
Symbol description No Data
References Cell. 1994 Dec 30;79(7):1267-76.


Targeted and natural (piebald-lethal) mutations of endothelin-B receptor gene produce megacolon associated with spotted coat color in mice.
Targeted and natural (piebald-lethal) mutations of endothelin-B receptor gene produce megacolon associated with spotted coat color in mice.
Targeted and natural (piebald-lethal) mutations of endothelin-B receptor gene produce megacolon associated with spotted coat color in mice.
Targeted and natural (piebald-lethal) mutations of endothelin-B receptor gene produce megacolon associated with spotted coat color in mice.
Research applications Fluorescent Proteins/lacZ System,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
The DEPOSITOR of BIOLOGICAL RESOURCE must obtain a consent on use of it from the DEVELOPER of the Ednrbtm1Ywa and Tg(DBH-Ednrb)Ywa (Professor Masashi Yanagisawa, University of Tsukuba (yanagisawa.masa.fu@u.tsukuba.ac.jp) and the DEVELOPER of Tg(Dct-lacZ)A12Jkn (Professor Ian J.Jackson, Western General Hospital(Edinburgh, UK)(ian.jackson@igmm.ed.ac.uk) before providing the mice to the RECIPIENT.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Kato, Masashi (Chubu University College of Life and Health Sciences) Kato, Masashi
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :