RBRC05861, B6.Cg-Tg(CAG/Su9-DsRed2,Acr3-EGFP)RBGS002Osb

RBRC No. RBRC05861
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(CAG/Su9-DsRed2,Acr3-EGFP)RBGS002Osb
Former Common name B6-RBGS-002, B6-CAGsu9-DsRed2/Acr3-GFP Tg#RBGS002
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2003. C57BL/6N background.
Strain description Trangenic mice expressing GFP in sperm acrosome from acr3-EGFP and RFP in sperm mitochondoria from CAG/su9-DsRed2. The dual fluorescent sperm show normal fertilizing ability and can be observed through uterine and oviductal walls under excitation light. Acrosome reacted sperm and acrosome intact sperm are easily distinguishable from each other by red fluorescence. Acr3-EGFP, in which EGFP with a proacrosin signal peptide and proacrosin N-terminal peptide were connected to the acrosin promoter. CAG/su9-DsRed2, in which a RFP(DsRed2) is connected to the CAG promoter with a mitochondrial import signal sequence of Atp5g1(su9).
Colony maintenance Carrier x Noncarrier [C57BL/6NJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Su9
Symbol name mitochondria localization signal of mouse Atp5g1[ATP synthase Fo complex subunit 9 (su9)]
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
2 Symbol DsRed2
Symbol name Red Fluorescent Protein (Discosoma sp.)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse proacrosin promoter
3 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Developmental Biology Research,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Exp. Anim., 59, 105-107 (2010).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
2 Genotyping protocol <PCR>
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :