RBRC05873, C57BL/6-Spata4<tm1a(KOMP)Osb>/3G

RBRC No. RBRC05873
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6-Spata4<tm1a(KOMP)Osb>/3G
Former Common name Spata4 KO, B6-Spata4 floxed#3G
H-2 Haplotype No Data
ES cell line EGR-G101 [C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Yoshitaka Fujihara and Dr. Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2012.
Strain description Spata4 gene floxed mice (Knockout-first).
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6NJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Ires
Symbol name internal ribosomal entry site (EMCV)
Chromosome 8
Common name internal ribosomal entry site from EMCV
Symbol description No Data
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 8
Common name neo; neomycin;
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 8
Common name No Data
Symbol description No Data
4 Symbol Spata4
Symbol name spermatogenesis associated 4
Chromosome 8
Common name No Data
Symbol description No Data
5 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 8
Common name No Data
Symbol description No Data
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 8
Common name No Data
Symbol description No Data
References Unpublished

Research applications Cre/loxP system,
Developmental Biology Research,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Proc. Natl. Acad. Sci. U S A., 113, 7704-7710 (2016).
Using the BIOLOGICAL RESOURCE is limited to the academic research of academic institutions. The RECIPIENT must contact in advance the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested.
Additional information
1 Lab HP
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months