Strain Data Sheet

RBRC05889

Strain Information

Image
BRC No.RBRC05889
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Dpysl2<tm1Gosh>
Former Common nameCRMP2KI
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Naoya Yamashita, Yokohama City University Graduate School of Medicine, Katsuhiko Mikoshiba, RIKEN Brain Science Institute in 2006. A floxed neo cassette remains inserted upstream of exon 13. The mutant mice were backcrossed to C57BL/6 over six times.
Strain descriptionCRMP2 (Dpysl2) Ser522Ala mutation knock-in mice.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
ReferencesJ. Neurosci., 32, 1360-1365 (2012). 22279220

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dpysl2dihydropyrimidinase-like 214Dpysl2<tm1Gosh>targeted mutation 1, Yoshio Goshima

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP14loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP14loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)14mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAphage P1 loxP sites, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter), mouse Dpysl2 genomic DNA
Research applicationCre/loxP system
Neurobiology Research
Specific Term and ConditionsUse of the BIOLOGICAL RESOURCE shall be limited to a collaborative research requiring co-authorship of RIKEN CBS Katsuhiko Mikoshiba, Ph.D. (DEPOSITOR). Waseda University Center for Advanced Biomedical Sciences Yoshio Goshima, Ph.D., Yokohama City University Yoshiro Goshima, Ph.D. in publication.
DepositorKatsuhiko Mikoshiba (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data