Strain Information | |
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Image | |
BRC No. | RBRC05889 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129P2-Dpysl2<tm1Gosh> |
Former Common name | CRMP2KI |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Developed by Naoya Yamashita, Yokohama City University Graduate School of Medicine, Katsuhiko Mikoshiba, RIKEN Brain Science Institute in 2006. A floxed neo cassette remains inserted upstream of exon 13. The mutant mice were backcrossed to C57BL/6 over six times. |
Strain description | CRMP2 (Dpysl2) Ser522Ala mutation knock-in mice. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6J(Crlj)] |
References | J. Neurosci., 32, 1360-1365 (2012). 22279220 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP14loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP14loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)14mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | phage P1 loxP sites, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter), mouse Dpysl2 genomic DNA |
Research application | Cre/loxP system Neurobiology Research |
Specific Term and Conditions | Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research requiring co-authorship of RIKEN CBS Katsuhiko Mikoshiba, Ph.D. (DEPOSITOR). Waseda University Center for Advanced Biomedical Sciences Yoshio Goshima, Ph.D., Yokohama City University Yoshiro Goshima, Ph.D. in publication. |
Depositor | Katsuhiko Mikoshiba (RIKEN) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |