RBRC05892, B6.129P2-Reck<tm1Ito>/Noda

RBRC No. RBRC05892
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Reck<tm1Ito>/Noda
Former Common name Recktm1(neo)Noda, global Reck knockout
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Chiaki Tkahashi, Regina M. Sasahara, Junseo Oh, Kyoto University Graduate School of Medicine, Shigeyoshi Itohara, RIKEN Brain Research Institute. E14 ES cells were used. The mutant mice were crossed to C57BL/6.
Strain description Reck gene knockout mice. Exon 1 of the Reck gene was replaced with a PGK-neo cassette. Homozygous mutant mice die around E10.5 with defects in collagen fibrils, basal lamina and vascular development.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJmsSlc] Homozygous mutant mice are embryonic lethal.
Health Report
Gene Details
Promoter No Data
1 Symbol Reck
Symbol name reversion-inducing-cysteine-rich protein with kazal motifs
Chromosome 4
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 4
Common name No Data
Symbol description No Data
References Nat Neurosci. 2007 Jul;10(7):838-45. Epub 2007 Jun 10.

RECK modulates Notch signaling during cortical neurogenesis by regulating ADAM10 activity.
RECK modulates Notch signaling during cortical neurogenesis by regulating ADAM10 activity.
Research applications Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Cell, 107, 789-800 (2001).
1. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an
acknowledgment to the DEPOSITOR is requested.
2. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
3. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain approval from the DEPOSITOR using the Approval Form.
4. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT shall reach agreement on terms and conditions of use of it with DEPOSITOR and shall obtain a prior written consent from the DEPOSITOR.
5. The RECIPIENT shall contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Noda, Makoto (Graduate School of Medicine, Kyoto University) Noda, Makoto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months