RBRC05893, B6.Cg-Tg(AKT/Ert2,EGFP)1Nkn

RBRC No. RBRC05893
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(AKT/Ert2,EGFP)1Nkn
Former Common name Akt-Mer Tg, B6-AKT/Ert2, GFP Tg
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Toru Nakano, Graduate School of Medicine, Osaka University. The mice were backcrossed to C57BL/6N over 8 times by Shigeo Koyasu, School of Medicine, Keio University.
Strain description AKT-Mer transgenic mice. These transgenic mice express a fusion protein composed of a myristoylated constitutively active form of human Akt and the ligand-binding domain of a mutant estrogen receptor under the control of the CAG promoter. The Akt-Mer was also fused to IRES-EGFP, can be monitored Akt-Mer expression by GFP fluorescence.
Colony maintenance Carrier x Noncarrier [C57BL/6NCrSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol ERT2
Symbol name estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol HA
Symbol name human influenza virus HA (hemagglutinin) tag sequence
Chromosome UN
Common name No Data
Symbol description No Data
4 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
5 Symbol AKT
Symbol name thymoma viral proto-oncogene (human)
Chromosome UN
Common name No Data
Symbol description No Data
References Oncogene. 2007 Feb 12;.

Akt activation induces epidermal hyperplasia and proliferation of epidermal progenitors.
Akt activation induces epidermal hyperplasia and proliferation of epidermal progenitors.
Research applications Cancer Research,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER, Professor Toru Nakano of Osaka University.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months