RBRC05917, B6.129S2-Mir200b<tm1.1Osb> Mir429<T30C>

RBRC No. RBRC05917
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129S2-Mir200b<tm1.1Osb> Mir429<T30C>
Former Common name miR-DKO, Mir200b<tm1.1Osb> Mir429<T30COsb>/39
H-2 Haplotype No Data
ES cell line D3 [129S2/SvPas]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hidetoshi Hasuwa and Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2003. The mutant mice were backcrossed to C57BL/6N over 7 times.
Strain description miR200b knockout mice having a point mutation in miR429. Homozygous mutant females show a severe infertility due to abnormal ovulation.
Colony maintenance Heterozygote x Wild-type[C57BL/6NJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Mir200b
Symbol name microRNA 200b
Chromosome 4
Common name No Data
Symbol description No Data
2 Symbol Mir429
Symbol name microRNA 429
Chromosome 4
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 4
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Science, 341, 71-73 (2013).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Ikawa, Masahito (Osaka university) Ikawa, Masahito
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months