RBRC05918, B6.Cg-Tg(TOPGAL)

RBRC No. RBRC05918
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(TOPGAL)
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takuya Murata, RIKEN BioResource center in 2012. The mice were backcrossed to C57BL/6 from TOP-GAL mice of ICR background (RBRC02228).
Strain description Ins-TOPGAL transgenic mice for visualizing Wnt signal pathway. The transgene contains the nLacZ gene under the control of a TOPFLASH promoter (6x TCF binding motifs upstream of a TK promoter). The transgene construct was flanked by the insulator core elements.
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl] Tg mice are viable and fertile.
Health Report
Gene Details
Promoter No Data
1 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter TOPFLUSH promoter
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome UN
Common name No Data
Symbol description No Data
References Genesis. 2007 Feb;45(2):90-100.

GFP transgenic mice reveal active canonical Wnt signal in neonatal brain and in adult liver and spleen.
GFP transgenic mice reveal active canonical Wnt signal in neonatal brain and in adult liver and spleen.
Research applications Developmental Biology Research,
Fluorescent Proteins/lacZ System,
Genetics Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
(will be announced)
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Gondo, Yoichi (RIKEN, BioResource Center) Gondo, Yoichi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Type XVII collagen coordinates proliferation in the interfollicular epidermis. Elife6: (2017).(28693719)
Watanabe M, Natsuga K, Nishie W, Kobayashi Y, Donati G, Suzuki S, Fujimura Y, Tsukiyama T, Ujiie H, Shinkuma S, Nakamura H, Murakami M, Ozaki M, Nagayama M, Watt FM, Shimizu H.