RBRC05931, C57BL/6-Gt(ROSA)26Sor<tm1.1(mCherry-MBD)Osb>

RBRC No. RBRC05931
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6-Gt(ROSA)26Sor<tm1.1(mCherry-MBD)Osb>
Former Common name MethylRO (Methylation probe in ROSA26 locus)
H-2 Haplotype No Data
ES cell line EGR-G101 [C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Jun Ueda and Kazuo Yamagata, Research Institute for Microbial Diseases, Osaka University. EGR-G101 ES cells derived from C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb were used. Frt flanked neo was removed by transient expression of FLP recombinase in fertilized eggs of conditional MethylRO mice.
Strain description ROSA26 mCherry-MBD knock-in mice (MethylRO).
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6NJcl]
Homozygous mutant mice show no obvious abnormality.
Health Report
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome No Data
Common name No Data
Symbol description No Data
3 Symbol MBD1
Symbol name methyl-CpG binding domain protein 1 (human)
Chromosome No Data
Common name No Data
Symbol description No Data
4 Symbol SA
Symbol name Splice acceptor (adenovirus)
Chromosome No Data
Common name No Data
Symbol description No Data
5 Symbol mCherry
Symbol name Red Fluorescent Protein (Discosoma sp.)
Chromosome No Data
Common name No Data
Symbol description No Data
References Dev Biol. 2006 Dec 24;.
Dev Biol. 2007 Dec 1;312(1):419-26. Epub 2007 Sep 29.

Time-lapse and retrospective analysis of DNA methylation in mouse preimplantation embryos by live cell imaging.
Time-lapse and retrospective analysis of DNA methylation in mouse preimplantation embryos by live cell imaging.
Research applications Cancer Research,
Cell Biology Research,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
will be announced.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 No Data
Depositor Yamagata, Kazuo (Osaka university) Yamagata, Kazuo
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months