Strain Data Sheet

RBRC05949

Strain Information

Image
BRC No.RBRC05949
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Rbm3<tm1Ttg>
Former Common nameB6-RBM3 KO, B6-Rbm3 KO
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Tadatsugu Taniguchi, Graduate School of Medicine, University of Tokyo in 2011. E14.1 ES cell derived. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionRbm3 gene knockout mice. The region containing exon 4 and 5 and part of exons 3 and 6 of Rbm3 gene were replaced with a PGK-neo cassette. Homozygous and hemizygous mutant mice show no obvious abnormality.
Colony maintenanceHomozygote (female) x Hemizygote (female) [or Crossing to C57BL/6JJcl]
ReferencesBiochem. Biophys. Res. Commun., 411, 7-13 (2011). 21684257

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rbm3RNA binding motif protein 3XRbm3<tm1Ttg>targeted mutation 1, Tadatsugu Taniguchi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)Xmouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Rbm3 genomic DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT shall inform the DEPOSITOR about the purpose of the use of the BIOLOGICAL RESOURCE and shall limit the use within the scope of the purpose described in this Agreement. The RECIPIENT shall inform the DEPOSITOR about the results obtained by the use of the BIOLOGICAL RESOURCE.
DepositorTadatsugu Taniguchi (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data