Strain Data Sheet

RBRC05977

Strain Information

Image
BRC No.RBRC05977
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Mmp14<tm2Ski>
Former Common nameMT1-MMP(MMP14) knockout mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Motoharu Seiki and Motoya Katsuki, Institute of Medical Science, University of Tokyo. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6J.
Strain descriptionT1-MMP (Mmp14) gene knockout mice (lacZ knock-in mice). Exons 1-5 were replaced with a NLS-lacZ cassette. Homozygous mutant mice die within 2-3 weeks after birth.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesGenes Cells., 14, 617-626 (2009). 19371380
J. Cell Sci., 120, 1607-1614 (2007). 17405818

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mmp14matrix metallopeptidase 14 (membrane-inserted)14Mmp14<tm2Ski>targeted mutstion 2, Motoharu Seiki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
gptguanine phosphoribosyltransferase14gptmouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)14

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)14herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)14nls

Ordering Information

Donor DNASV40 nuclear localization signal (NLS), E. coli LacZ, mouse Phosphoglcerate kinase promoter (PGK promoter), E. coli gpt, HSV Thymidine kinase promoter, E.coli neomycin resistance gene, SV40 PolyA signal
Research applicationDermatology Research
Developmental Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells., 14, 617-626 (2009).
DepositorMotoharu Seiki (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data