RBRC05977, B6.129P2-Mmp14<tm2Ski>

RBRC No. RBRC05977
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Mmp14<tm2Ski>
Former Common name MT1-MMP(MMP14) knockout mice
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Motoharu Seiki and Motoya Katsuki, Institute of Medical Science, University of Tokyo. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6J.
Strain description T1-MMP (Mmp14) gene knockout mice (lacZ knock-in mice). Exons 1-5 were replaced with a NLS-lacZ cassette. Homozygous mutant mice die within 2-3 weeks after birth.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Mmp14
Symbol name matrix metallopeptidase 14 (membrane-inserted)
Chromosome 14
Common name No Data
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 14
Common name No Data
Symbol description No Data
3 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome 14
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 14
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
5 Symbol gpt
Symbol name guanine phosphoribosyltransferase
Chromosome 14
Common name No Data
Symbol description No Data
References J Cell Sci. 2007 Apr 12;.

Crosstalk between neovessels and mural cells directs the site-specific expression of MT1-MMP to endothelial tip cells.
Crosstalk between neovessels and mural cells directs the site-specific expression of MT1-MMP to endothelial tip cells.
Research applications Dermatology Research,
Developmental Biology Research,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genes Cells., 14, 617-626 (2009).
Additional information
1 No Data
Depositor Seiki, Motoharu (The Institute of Medical Science, The University of Tokyo) Seiki, Motoharu
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months