RBRC06041, ICR.Cg-Hcn4<tm1(tTA)>/2Kyo

RBRC No. RBRC06041
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name ICR.Cg-Hcn4<tm1(tTA)>/2Kyo
Former Common name ICR-Tet-HCN4#1, ICR-Hcn4/tTA KI#2
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takahiro Ishii, Noriyuki Nakashima and Harunori Ohmori, Faculty of Medicine, Kyoto University. TT2 ES cells were used. The mutant mice were backcrossed to C57BL/6 and ICR over 20 times, respectively. C57BL/6 background (line 1: RBRC06038, line 2: RBRC06039), ICR background (line 1: RBRC06040, line 2: RBRC06040).
Strain description Hcn4 tTA knock-in mice.
Colony maintenance Heterozygote x Wild-type [or Crossing to Slc:ICR] Homozygous mutant mice may be embryonic lethal.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol tTA
Symbol name tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)
Chromosome 9
Common name E. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain
Symbol description No Data
2 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome 9
Common name No Data
Symbol description No Data
3 Symbol GH
Symbol name human growth hormone gene
Chromosome 9
Common name No Data
Symbol description No Data
4 Symbol URA3
Symbol name Orotidine-5′-phosphate (OMP) decarboxylase (yeast)
Chromosome 9
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 9
Common name No Data
Symbol description No Data
Promoter E. coli tet operator, cytomegalovirus CMV promoter
6 Symbol Hcn4
Symbol name hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Chromosome 9
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
7 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 9
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Tet system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Physiol., 591, 1749-1769 (2013).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR/DEVELOPER(S).
Additional information
1 No Data
Depositor Ohmori, Harunori (Graduate School of Medicine, Kyoto University) Ohmori, Harunori
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months