RBRC06047, B6.Cg-Hes1<tm1Imayo>

RBRC No. RBRC06047
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Hes1<tm1Imayo>
Former Common name B6-Hes1-floxed ; B6-Hes1 floxed
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2007. TT2 ES cells were used. The mutant mice were crossed to C57BL/6 for 6 times.
Strain description Hes1 gene floxed mice. LoxP sequences were knocked into the first intron and downstream of 3’UTR of Hes1 gene for conditional knock-out study. PGK-Neo sequence was removed.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Hes1
Symbol name hairy and enhancer of split 1 (Drosophila)
Chromosome 16
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 16
Common name No Data
Symbol description No Data
References Development. 2008 Aug;135(15):2531-41. Epub 2008 Jun 25.

Hes genes and neurogenin regulate non-neural versus neural fate specification in the dorsal telencephalic midline.
Research applications Cre/loxP system,
Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Development. 2008 135(15):2531-41.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :