Strain Information | |
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Image | |
BRC No. | RBRC06153 |
Type | Transgene |
Species | Mus musculus |
Strain name | B6.Cg-Tg(TOPGAL) Ctnnb1<Rgsc1880> |
Former Common name | TOPGAL;Ctnnb1/C429S (B6), B6-TOPGAL Ctnnb1/C429S |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Takuya Murata and Yoichi Gondo, RIKEN BioResource center in 2012. C57BL/6J background. |
Strain description | Ins-TOPGAL and Ctnnb1 C429S mutant mice. Ctnnb1 C429S mutant mice were generated by ENU mutagenesis. C492S homozygous mutant mice are infertile. Ins-TOPGAL transgenic mice are useful for visualizing Wnt signal pathway. The transgene contains the nLacZ gene under the control of a TOPFLASH promoter (6x TCF binding motifs upstream of a TK promoter). The transgene construct was flanked by the insulator core elements. |
Colony maintenance | Tg: Carrier x Noncarrier [C57BL/6JJcl]; Ctnnb1: Heterozygote x Wild-type [C57BL/6JJcl] |
References | Nat. Rev. Genet., 9, 803-810 (2008). 18781157 Sci. Rep., 4, 6959 (2014). 25376241 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)UNTOPFLUSH promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)UNnls |
Ordering Information | |
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Donor DNA | TCF binding site from TOPFLUSH vector (Upstate) , HSV TK promoter, E. coli LacZ, SV40-late-poly A, chicken β-globin HS4 insulater, Simian virus 40 Large T antigen nuclear localization signal (nls) |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Yoichi Gondo (RIKEN GSC) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Mouse of the Month Nov 2014 |
BRC mice in Publications |
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