RBRC06197, B6.129S4-Cckar<tm1Kym>

RBRC No. RBRC06197
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129S4-Cckar<tm1Kym>
Former Common name CCKAR KO, B6-Cckar KO
H-2 Haplotype No Data
ES cell line J1 [129S4/SvJae]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Soichi Takiguchi and Akihiro Funakoshi, National Kyusu Cancer Center, Tetsuo Noda, The Cancer Institute, Japanese Foundation for Cancer Research and Kyoko Miyasaka, Tokyo Metropolitan Institute of Gerontology. J1 ES cells were used. The mutant mice were backcrossed to C57BL/6J.
Strain description Cckar gene knockout mice. Part of exon 1 and all of exon 2 were replaced with a NLS-lacZ neo cassette. Homozygous mutant mice are viable and fertile.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJmsSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol Cckar
Symbol name cholecystokinin A receptor
Chromosome 5
Common name No Data
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 5
Common name No Data
Symbol description No Data
3 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome 5
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 5
Common name No Data
Symbol description No Data
References

Research applications Fluorescent Proteins/lacZ System,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Pancres, 24, 276-283 (2002).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Miyasaka, Kyoko (Tokyo Kasei University) Miyasaka, Kyoko
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months