RBRC06209, B6.Cg-Gt(ROSA)26Sor<tm1(Dte/3xHA)Hmd>

RBRC No. RBRC06209
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Gt(ROSA)26Sor<tm1(Dte/3xHA)Hmd>
Former Common name ROSA26-3HA Dte KI
H-2 Haplotype No Data
ES cell line G4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Tetsuya Nakamura and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2010. G4 ES cells were used. C57BL/6 and 129 mixed background.
Strain description Dte-3xHAtag ROSA26 conditional knock in mice. PGK-neo cassette with SV40 ployA and Dte (Dand5)-3xHA were inserted into the Gt(ROSA)26Sor. The Dte-3xHA expression can be generated by crossing with tissue-specific Cre mice.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Dand5
Symbol name DAN domain family, member 5
Chromosome 6
Common name Dte
Symbol description No Data
2 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol HA
Symbol name human influenza virus HA (hemagglutinin) tag sequence
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol SA
Symbol name Splice acceptor (adenovirus)
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
7 Symbol polyA
Symbol name SV40 virus polyA signal sequence
Chromosome 6
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
8 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months