Strain Information | |
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Image | |
BRC No. | RBRC06211 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129-Piga<tm1> |
Former Common name | Piga floxed, B6-Piga floxed |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | Developed by Junji Takeda and Taroh Kinoshita, Research Institute for Microbial Diseases, Osaka University in 1997. R1 ES cells derived were used for the generation of floxed mice. The mutant mice were backcrossed to C57BL/6J over 10 times. |
Strain description | Piga gene floxed mice. A loxP site was inserted between exons 5 and 6, and a loxP flanked Tk-neo cassette was inserted after the exon 5. |
Colony maintenance | Heterozygote, Hemizygote x Wild-type [C57BL/6JJcl] |
References | Proc. Natl. Acad. Sci. USA, 94, 7400-7405 (1997). 9207103 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Pigaphosphatidylinositol glycan anchor biosynthesis, class AXPiga<tm1Tak>targeted mutation 1, Junji Takeda Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPXloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPXloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPXloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)Xherpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Ordering Information | |
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Donor DNA | Phage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Piga genomic DNA |
Research application | Cre/loxP system Hematological Research Mouse Models for Human Disease |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Taroh Kinoshita (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |