RBRC06247, B6.Cg-Gab2<tm5.1Thir>

RBRC No. RBRC06247
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Gab2<tm5.1Thir>
Former Common name Gab2 Y441F/Y465F/Y573F/Y603F/Y632F KI, Gab2 deltaSHP2/deltaPI3K KI, B6-Gab2/dSHP2/dPI3K K
H-2 Haplotype No Data
ES cell line M1 [(C57BL/6 x 129)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Keigo Nishida, RIKEN Center for Allergy and Immunology. M1 ES cells derived from (C57BL/6 x 129)F1 were used for the generation of knockout mice. A floxed neo cassette was removed by crossing with B6-CAG-cre mice. C57BL/6 and 129 mixed background.
Strain description Gab2 mutant gene knock-in mice. A cDNA encoding 89-665 amino acids was inserted into exon 2 of Gab2 gene. The cDNAs encoded wild-type (WT) (RBRC06244), Y603F/Y632F mutant abolishing the SHP2-binding domain (RBRC06245), Y441F/Y465F/Y573F mutant abolishing PI3K-binding domain (RBRC06246) and Y441F/Y465F/Y573F/Y603F/Y632F mutant abolishing the SHP2 and PI3K-binding domains (RBRC06247) mutant, respectively.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name mouse Gab2 Y441F/Y465F/Y573F/Y603F/Y632F mutant cDNA encoding amino acid 89-665
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 7
Common name No Data
Symbol description No Data
3 Symbol Gab2
Symbol name growth factor receptor bound protein 2-associated protein 2
Chromosome 7
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
References

Research applications Cell Biology Research,
Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Immunol., 187, 932-941 (2011).
Additional information
1 No Data
Depositor Nishida, Keigo (RIKEN Center for Integrative Medical Sciences (IMS)) Nishida, Keigo
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months