RBRC06303, B6.Cg-Malat1<tm1.1Shna>/ShnaRbrc

RBRC No. RBRC06303
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Malat1<tm1.1Shna>/ShnaRbrc
Former Common name Malat1 KO, B6-Malat1 KO, NEAT2(lacZ)(Acc.No.CDB0837K)
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Shinichi Nakagawa, RIKEN Advanced Science Institute. TT2 ES cells were used to generate the knockout mice. A FRT-flanked neo cassette was removed by crossing with FLP mice. The mutant mice were backcrossed to C57BL/6N.
Strain description Malat1 ncRNA knock-out mice. Homozygous mutant mice are viable and fertile.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 19
Common name No Data
Symbol description No Data
2 Symbol Malat1
Symbol name metastasis associated lung adenocarcinoma transcript 1 (non-coding RNA)
Chromosome 19
Common name No Data
Symbol description No Data
3 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 19
Common name No Data
Symbol description No Data
4 Symbol lox71
Symbol name mutant lox sequence
Chromosome 19
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 19
Common name No Data
Symbol description No Data
References

Research applications Cell Biology Research,
Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
(1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No.CDB0837K and Web. Site (http://www.cdb.riken.jp/arg/mutant%20mice%20list.html) is requested. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested (RNA. 2012 18(8):1487-99.). (3) Please inform the DEPOSITOR (mutant@cdb.riken.jp) about the paper when published.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor RIKEN, CDB (RIKEN Center for Developmental Biology) RIKEN, CDB
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :