RBRC06313, B6.129-Sparc<tm1Hwe>

RBRC No. RBRC06313
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129-Sparc<tm1Hwe>
Former Common name B6-Sparc KO
H-2 Haplotype No Data
ES cell line CCE/EK.CCE [129S/SvEv-Gpi1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Chin Chen Howem The Wistar Institute. CCB ES cells were used to generate the knockout mice. The mutant mice were maintained by Yasumasa Kato, Ohu university School of Dentistry through Helene Sga, Benaroya Research Institute at Virginia. C57BL/6 congenic.
Strain description Sparc (osteonectin, BM-40) gene knockout mice. Exon 4 of Sparc gene was replaced with MC1-neo and Pol2-neo tandem cassettes. Homozygous mutant mice exhibit cataracts, abnormal skeletal morphology and osteopenia (viable and fertile).
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol Sparc
Symbol name secreted acidic cysteine rich glycoprotein
Chromosome 11
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name No Data
Symbol description No Data
Promoter mouse RNA polymerase II promoter
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name No Data
Symbol description No Data
References

Research applications Cancer Research,
Cell Biology Research,
Developmental Biology Research,
Hematological Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Invest. Ophthalmol. Vis. Sci., 39, 2674-2680 (1998 ).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Kato, Yasumasa (Kanagawa Dental College) Kato, Yasumasa
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months